Methods in Molecular Biology Essential Genes and Genomes: Methods and Protocols, Book 2377, (Paperback)

<p><b>1. A Method to Map Gene Essentiality of Human Pluripotent Stem Cells by Genome-Scale CRISPR Screens with Inducible Cas9</b></p> <p> Barbara Mair, Michael Aregger, Amy H.Y. Tong, Katherine S.K. Chan, and Jason Moffat</p> <p> </p> <p><b>2. CRISPR/Cas9 Screening to Identify Conditionally Essential Genes in Human Cell Lines</b></p> <p> Kimberly S. Huggler, Nicholas J. Rossiter, Kyle M. Flickinger, and Jason R. Cantor</p> <p> </p> <p><b>3. </b><b>Target Identification of Small Molecules Using Large-Scale CRISPR-Cas Mutagenesis Scanning of Essential Genes</b></p> <p> Bert Kwanten, Jasper Neggers, and Dirk Daelemans</p> <p> </p> <p><b>4. Design and Generation of a CRISPR Interference System for Genetic Repression and Essential Gene Analysis in the Fungal Pathogen <i>Candida albicans</i></b></p> <p> Lauren Wensing and Rebecca S. Shapiro</p> <p> </p> <p><b>5. Identification of Essential Genes Using Sequential CRISPR and siRNA Screens</b></p> <p> Luke DeHart, Oliver P. Yockey, and Jesse Bakke</p> <p> </p> <p><b>6. A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Vertebrate Cell Lines</b></p> <p> Kohei Nishimura and Tatsuo Fukagawa</p> <p> </p> <p><b>7. New Method for Genome-Scale Functional Genomics Study in Bacteria with Superior Performance: CRISPR Interference Screen</b></p> <p> Xihao Liao, Xin-Hui Xing, and Chong Zhang</p> <p> </p> <p><b>8. A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in Cyanobacteria</b></p> <p> Ju-Yuan Zhang, Tian-Cai Niu, Gui-Ming Lin, and Cheng-Cai Zhang</p> <p> </p> <p><b>9. A Method of Transposon Insertion Sequencing in Comprehensively Identifying <i>Vibrio vulnificus</i> Genes Required for Growth in Human Serum</b></p> <p> Miguel Carda-Diéguez and Carmen Amaro</p> <p></p> <p><b>10. The Use of Tn-Seq and the FiTnEss Analysis to Define the Core Essential Genome of <i>Pseudomonas aeruginosa</i></b></p> <p> Bradley E. Poulsen, Anne E. Clatworthy, and Deborah T. Hung</p> <p> </p> <p><b>11. Analysis of <i>Escherichia coli</i> K1 Virulence Genes by Transposon-Directed Sequencing</b></p> <p> Alex J. McCarthy and Peter W. Taylor</p> <p> </p> <p><b>12. Using Genome Scale Mutant Libraries to Identify Essential Genes</b></p> <p> Kevin S. Myers, Piyush Lal, Daniel R. Noguera, and Timothy J. Donohue</p> <p> </p> <p><b>13. Identification and Analysis of Essential Genes in <i>Streptococcus mutans</i></b></p>

• Methods in Molecular Biology Essential Genes and Genomes: Methods and Protocols, Book 2377, (Paperback)
• Author: Ren Zhang
• ISBN: 9781071617229
• Format: Paperback
• Publication Date: 2022-10-29
• Page Count: 434